کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4419333 1618935 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Octylphenol induced gene expression in testes of Frog, Rana chensinensis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Octylphenol induced gene expression in testes of Frog, Rana chensinensis
چکیده انگلیسی


• A subtractive cDNA library was constructed in testes of frogs OP exposure.
• 134 genes were produced from the subtractive libraries randomly.
• OP may induce immune response, and affect steroid synthesis and spermatogenesis.

Octylphenol (OP) is an endocrine-disrupting chemical (EDC), which can disrupt the reproductive system. To understand the effect of OP, a subtractive cDNA library was constructed using suppression subtractive hybridization (SSH) to identify alterations of gene transcription in the testes of the frog Rana chensinensis after OP exposure. Two hundred positive clones were selected and 134 sequences of gene fragments were produced from the subtractive library randomly. These genes were identified to be involved in metabolic process, cellular process, biological regulation, stimulus, immune system and female pregnancy process. In order to verify the efficiency of the subtractive cDNA library, PSG9 and PAPP-A were analyzed further as two representatives of differentially expressed transcription genes using semi-quantitative RT-PCR. Our result was the first successful construction of the subtractive cDNA library in frog testes after OP treatment. Based on this cDNA library, OP was shown to affect multiple physiological processes including inducing immune response, disrupting the steroid hormone synthesis and influencing spermatogenesis in the testis by up-regulation of specific genes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 128, June 2016, Pages 75–82
نویسندگان
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