Ecotoxicological evaluation of swine manure disposal on tropical soils in Brazil

• The toxicity of swine manure for E. andrei was tested in tropical Brazilian soils.
• In Ultisol, Oxisol and Tropical Artificial Soil the waste did not cause negative impacts.
• Only in the Entisol the swine manure caused deleterious effects on E. andrei.
• Soil parameters, such as texture, organic matter can alter the toxicity for earthworms of swine manure.
• The ecotoxicological assays can be used to define the toxicity grade of swine manure in soils.

Swine production in Brazil results in a great volume of manure that normally is disposed of as agricultural fertilizer. However, this form of soil disposal, generally on small farms, causes the accumulation of large amounts of manure and this results in contaminated soil and water tables. To evaluate the effects of increasing concentrations of swine manure on earthworms, several ecotoxicological tests were performed using Eisenia andrei as test organism in different tropical soils, classified respectively as Ultisol, Oxisol, and Entisol, as well as Tropical Artificial Soil (TAS). The survival, reproduction and behavior of the earthworms were evaluated in experiments using a completely randomized design, with five replications. In the Ultisol, Oxisol and TAS the swine manure showed no lethality, but in the Entisol it caused earthworm mortality (LOEC=45 m3 ha−1). In the Entisol, the waste reduced the reproductive rate and caused avoidance behavior in E. andrei (LOEC=30 m3 ha−1) even in lower concentrations. The Entisol is extremely sandy, with low cation exchange capacity (CEC), and this may be the reason for the higher toxicity on soil fauna, with the soil not being able to hold large amounts of pollutants (e.g. toxic metals), but leaving them in bioavailable forms. These results should be a warning of the necessity to consider soil parameters (e.g. texture and CEC) when evaluating soil contamination by means of ecotoxicological assays, as there still are no standards for natural soils in tropical regions. E. andrei earthworms act as indicators for a soil to support disposal of swine manure without generating harm to agriculture and ecosystems.