کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4419535 1618941 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Copper acutely impairs behavioral function and muscle acetylcholinesterase activity in zebrafish (Danio rerio)
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Copper acutely impairs behavioral function and muscle acetylcholinesterase activity in zebrafish (Danio rerio)
چکیده انگلیسی


• Acute copper exposure impaired locomotion of zebrafish.
• Acute copper exposure altered zebrafish natural behavior to avoid lit environments.
• Muscle AChE activity was inhibited by acute exposure to copper.
• Acute exposure to copper did not altered copper levels in zebrafish tissues.

Copper is a heavy metal found at relatively high concentrations in surface waters around the world. Copper is a micronutrient at low concentrations and is essential to several organisms. At higher concentrations copper can become toxic, which reveal the importance of studying the toxic effects of this metal on the aquatic life. Thus, the objective of this study was to evaluate the toxic effects of copper on the behavior and biochemical parameters of zebrafish (Danio rerio). Zebrafish were exposed for 24 h at a concentration of 0.006 mg/L Cu. After the exposure period, behavioral profile of animals was recorded through 6 min using two different apparatuses tests: the Novel Tank and the Light–Dark test. After behavioral testing, animals were euthanized with a solution of 250 mg/L of tricaine (MS-222). Brain, muscle, liver and gills were extracted for analysis of parameters related to oxidative stress and accumulation of copper in these tissues. Acetylcholinesterase (AChE) activity was determined in brain and muscle. Results showed acute exposure to copper induces significant changes in behavioral profile of zebrafish by changing locomotion and natural tendency to avoid brightly lit area. On the other hand, there were no significant effects on parameters related to oxidative stress. AChE activity decreased significantly in zebrafish muscle, but there were no significant changes in cerebral AChE activity. Copper levels in tissues did not increase significantly compared to the controls. Taken together, these results indicate that a low concentration of copper can acutely affect behavioral profile of adult zebrafish which could be partially related to an inhibition on muscle AChE activity. These results reinforce the need of additional tests to establishment of safe copper concentrations to aquatic organisms and the importance of behavioral parameters in ecotoxicological studies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 122, December 2015, Pages 440–447
نویسندگان
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