کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4420993 1618984 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of cda gene in bighead carp and its expression in response to microcystin-LR
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Identification of cda gene in bighead carp and its expression in response to microcystin-LR
چکیده انگلیسی

Microcystin-LR (MCLR) is a widespread cyanotoxin, which can influence genes transcription and cause nucleic acid damage in different organisms. To identify MCLR induced transcriptionally changed hepatic genes in bighead carp by subtractive suppression hybridization, we obtained the cDNA fragment of cda. Then we cloned its full-length cDNA, which encodes a cytidine deaminase (CDA). 3D structure prediction showed that the 3D structure and amino acid residues related to function sites of bighead carp CDA were highly conserved. Bighead carp CDA shared high identities with other CDA sequences, and evolved closely to non-mammalian CDAs. Bighead carp expressed cda in all tested tissues under normal situation, and changed its expression profile in a time inversely dependent and dose dependent manner to MCLR, so as to protect itself from MCLR induced toxic damage. These indicated that cda might be involved in anti-MCLR response, especially in the regulation of cytidine and dexocytidine metabolism pathway.


► We cloned a cda homolog in bighead carp.
► Bighead carp cda expressed in all tested tissues under normal condition.
► Bighead carp cda changed its expression profile in a time inversely dependent and dose dependent manner in response to MCLR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 79, 1 May 2012, Pages 206–213
نویسندگان
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