کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4421022 1308502 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Genotoxicity and oxidative stress biomarkers in Carassius gibelio as endpoints for toxicity testing of Ukrainian polluted river waters
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Genotoxicity and oxidative stress biomarkers in Carassius gibelio as endpoints for toxicity testing of Ukrainian polluted river waters
چکیده انگلیسی

This study aimed to assess oxidative stress and genotoxicity biomarkers in Prussian carp Carassius gibelio laboratory-exposed to water from polluted Ukrainian rivers in order to evaluate their usefulness as endpoints in a short-term bioassay for toxicity testing of freshwaters. The micronucleus (MN) test and the frequency of cells with double nuclei (DN) in erythrocytes and gill cells were used as indicators of chromosome aberrations and abnormalities in cell divisions, respectively. Cellular antioxidant defenses i.e. antioxidant enzyme activities (catalase, Se-dependent glutathione peroxidase, total glutathione peroxidase and glutathione-S-transferase) and oxidative damage, i.e. lipid peroxidation (measured as thiobarbituric acid reactive substances) in the fish liver were used as biomarkers of oxidative stress. Exposure to the polluted river water samples for 96 h resulted in significantly increased MN and DN frequencies, limited increases in antioxidant enzyme activities and no changes in lipid peroxidation. Results suggest that MN and DN frequencies in C gibelio are useful endpoints in a short-term bioassay for genotoxicity testing of environmental water samples in contrast to the oxidative stress biomarkers applied that showed low potential for assessing sublethal effects after a 96 h exposure.


► Carassius gibelio were exposed for 96 h to water from polluted Ukrainian rivers.
► Micronuclei and double nuclei frequencies increased indicating genotoxicity.
► Increases in antioxidant enzyme activities were limited.
► No changes were found in lipid peroxidation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 74, Issue 8, November 2011, Pages 2240–2244
نویسندگان
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