Hesperetin, a citrus flavonone, protects potentially cadmium induced oxidative testicular dysfunction in rats

The present study was aimed to evaluate the protective effect of hesperetin (Hp) on cadmium (Cd) induced oxidative testicular toxicity in rats. Subcutaneous administration of Cd (3 mg/kg body weight) for 21 days significantly elevated the levels of oxidative stress markers, Cd concentration in testis and lowered the levels of enzymatic, non-enzymatic antioxidants and membrane bound enzymes in the testicular tissue. Hp administrated orally along with Cd injection for 21 days, significantly revert back the status of oxidative stress markers, Cd concentration in testis, improved status of antioxidant markers and membrane bound enzymes in the testis to near normal level. The histopathological studies in the testis of rats also supported that Hp (40 mg/kg) markedly reduced the toxicity of Cd and preserved the normal histoarchitecture pattern of the testis. Thus, the results suggest that Hp acts as a potent antioxidative agent against Cd induced testicular toxicity in rats.

► Reduced the level of testicular lipid peroxidation and protein carbonylation.
► Increased the level of non-enzymatic antioxidant status of the testicular tissue.
► Increased the level of enzymatic antioxidant activity of the testicular tissue.
► Exhibited membrane stabilization and restored the levels of membrane bound ATPases.