کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4421410 1308512 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Real-time PCR-based prediction of gonad phenotype in medaka
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Real-time PCR-based prediction of gonad phenotype in medaka
چکیده انگلیسی

An important endpoint in aquatic bioassays for potential endocrine disrupting chemicals (EDCs) is the gonadal phenotype of exposed fish, with special interest in intersex and sex-reversed individuals. Traditionally, the assessment of gonad phenotype is done via histology, which involves specialized and time-consuming techniques. The method detailed here increases the efficiency of the analysis by first determining the relative expression of four genes involved in gonad development/maintenance in Japanese medaka (Oryzias latipes), and then by using principal component analysis, assigning a phenotype to each gonad based upon the gene expression data. The gonad phenotype and the sexual genotype, which can be determined in medaka, can then be compared to assess potential adverse effects of exposure to endocrine disrupting chemicals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 73, Issue 4, May 2010, Pages 589–594
نویسندگان
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