کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4421471 1308513 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Subcellular Cd distribution and its correlation with antioxidant enzymatic activities in wheat (Triticum aestivum) roots
چکیده انگلیسی

The subcellular partitioning of Cd in wheat root was obtained by differential centrifugation of root homogenates to quantify the amounts of Cd associated with five operationally defined subcellular fractions, namely Cd-rich granule (MRG), cellular debris, organelles, heat-denatured protein (HDP), and heat-stable protein (HSP). Their correlations were investigated with the changes in antioxidant enzymatic activities under Cd stress (0.01–9.68 μM) for 72 h. The results showed that the relative Cd distribution in detoxified fractions (MRG+HSP) was decreased with increasing [Cd], but in metal-sensitive fractions (HDP+organelles) was increased. Consequently, the content of superoxide dismutase (SOD) and catalase (CAT) was decreased. Additionally, an increase of the total antioxidant capacity (A-TOC) and a slight decrease of malondialdehyde (MDA) were also observed. Interestingly, the relative root elongation (RRL) and CAT were significantly correlated with the Cd-sensitive fractions. These results suggest that the Cd toxicity to wheat roots strongly depends on the subcellular Cd distribution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 74, Issue 4, May 2011, Pages 874–881
نویسندگان
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