کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4422054 1308529 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Hydroxyl radical generation and oxidative stress in Carassius auratus liver, exposed to pyrene
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Hydroxyl radical generation and oxidative stress in Carassius auratus liver, exposed to pyrene
چکیده انگلیسی

This paper studied the hydroxy radical generation and oxidative stress in the liver of goldfish Carassius auratus under the effect of pyrene. Fish were exposed to different concentrations (0.001, 0.005, 0.01, 0.05 and 0.1 mg/L) of pyrene for 10 days, with one group assigned as control. Based on the hyperfine splitting constants and shape of the electron paramagnetic resonance (EPR) spectrum, the free radical which was generated in fish liver was identified as hydroxyl radical (OH). The OH signal intensity showed a significant increase compared with the control. The changes of the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione-S-transferase (GST) were detected. The reduced glutathione (GSH) level decreased significantly while oxidized glutathione (GSSG) level was increased at higher concentration (0.005–0.1 mg/L), resulting in a decreased GSH/GSSG ratio, and the malondialdehyde (MDA) content increased significantly at 0.005–0.1 mg/L pyrene . The results clearly showed that C. auratus was subjected to oxidative stress and damage when exposed to pyrene.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ecotoxicology and Environmental Safety - Volume 71, Issue 2, October 2008, Pages 446–453
نویسندگان
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