Changes in hydrocarbon groups, soil ecotoxicity and microbiology along horizontal and vertical contamination gradients in an old landfarming field for oil refinery waste

Horizontal and vertical contaminant gradients in an old landfarming field for oil refinery waste were characterised with the aim to assess parallel changes in hydrocarbon groups and general, microbiological and ecotoxicological soil characteristics. In the surface soil polar compounds were the most prevalent fraction of heptane-extractable hydrocarbons, superseding GC–FID-resolvable and high-molar-mass aliphatics and aromatics, but there was no indication of their relatively higher mobility or toxicity. The size of the polar fraction correlated poorly with soil physical, chemical and microbiological properties, which were better explained by the total heptane-extractable and total petroleum hydrocarbons (TPH). Deleterious effects on soil microbiology in situ were observed at surprisingly low TPH concentrations (0.3%). Due to the accumulation of polar and complexed degradation products, TPH seems an insufficient measure to assess the quality and monitor the remediation of soil with weathered hydrocarbon contamination.

► Weathered hydrocarbon contamination and soil quality on landfarm site were studied.
► Silica fractionation of hydrocarbons separated aliphatics, aromatics and polars.
► Polar hydrocarbon metabolites had accumulated in the surface soil.
► Total hydrocarbons and TPH correlated with soil quality changes better than polars.
► Toxic response of soil microbial biomass and activity were seen at low TPH (<0.5%).