کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4425060 1619208 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction and application of a zinc-specific biosensor for assessing the immobilization and bioavailability of zinc in different soils
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
Construction and application of a zinc-specific biosensor for assessing the immobilization and bioavailability of zinc in different soils
چکیده انگلیسی

The inducibility and specificity of different czcRS operons in Pseudomonas putida X4 were studied by lacZ gene fusions. The data of β-glycosidase activity confirmed that the czcR3 promoter responded quantitatively to zinc. A zinc-specific biosensor, P. putida X4 (pczcR3GFP), was constructed by fusing a promoterless enhanced green fluorescent protein (egfp) gene with the czcR3 promoter in the chromosome of P. putida X4. In water extracts of four different soils amended with zinc, the reporter strain detected about 90% of the zinc content of the samples. Both the bioavailability assessment and the sequential extraction analysis demonstrated that the immobilization of zinc was highly dependent on the physico-chemical properties of soils. The results also showed that the lability of zinc decreased over time. It is concluded that the biosensor constitutes an alternative system for the convenient evaluation of zinc toxicity in the environment.


► A zinc-specific bacterial biosensor was developed.
► Four spiked soils were used to test the application of this biosensor.
► The bioavailable zinc in soil-water extracts decreased due to aging.
► The immobilization and speciation of zinc were highly dependent on the soil type.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental Pollution - Volume 164, May 2012, Pages 66–72
نویسندگان
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