کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4425468 | 1309104 | 2011 | 6 صفحه PDF | دانلود رایگان |
Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPARα–RXRα. It can be determined indirectly by immobilizing PPARα–RXRα–PFOS complex to another plate coated with PPARα antibody and subsequent measuring the level of PPARα–RXRα by using biotin-modified PPARα–RXRα probes–quantum dots–streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L−1 with linear range between 2.5 ng L−1 and 75 ng L−1. Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS.
► We developed a rapid and high-throughput bioassay for monitoring of PFOS in water.
► We detected the PFOS concentration of water samples by two methods.
► The bioassay is effective for evaluating PFOS contamination level.
Journal: Environmental Pollution - Volume 159, Issue 5, May 2011, Pages 1348–1353