کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
443900 692805 2015 15 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Context aware spatio-temporal cell tracking in densely packed multilayer tissues
ترجمه فارسی عنوان
ردیابی سلول های فضایی و زمانی بافتی در بافت های چند لایه چگالی بسته
کلمات کلیدی
ردیابی سلول، زمینه تصادفی محض، ارتباط داده فضایی و زمان، برچسب گذاری نمودار، تصویربرداری سلول زنده
موضوعات مرتبط
مهندسی و علوم پایه مهندسی کامپیوتر گرافیک کامپیوتری و طراحی به کمک کامپیوتر
چکیده انگلیسی


• We propose a cell tracker for 4D image stacks of densely packed multi-layer tissues.
• It exploits the tight spatial topology of neighboring cells as context information.
• It combines the spatial context with cells’ physical features through a CRF model.
• Feasibility and optimality of associations are ensured via a graph labeling problem.
• We show results on live-imaging confocal image stacks of Arabidopsis shoot meristem.

Modern live imaging technique enables us to observe the internal part of a tissue over time by generating serial optical images containing spatio-temporal slices of hundreds of tightly packed cells. Automated tracking of plant and animal cells from such time lapse live-imaging datasets of a developing multicellular tissue is required for quantitative, high throughput analysis of cell division, migration and cell growth. In this paper, we present a novel cell tracking method that exploits the tight spatial topology of neighboring cells in a multicellular field as contextual information and combines it with physical features of individual cells for generating reliable cell lineages. The 2D image slices of multicellular tissues are modeled as a conditional random field and pairwise cell to cell similarities are obtained by estimating marginal probability distributions through loopy belief propagation on this CRF. These similarity scores are further used in a spatio-temporal graph labeling problem to obtain the optimal and feasible set of correspondences between individual cell slices across the 4D image dataset. We present results on (3D + t) confocal image stacks of Arabidopsis shoot meristem and show that the method is capable of handling many visual analysis challenges associated with such cell tracking problems, viz. poor feature quality of individual cells, low SNR in parts of images, variable number of cells across slices and cell division detection.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Medical Image Analysis - Volume 19, Issue 1, January 2015, Pages 149–163
نویسندگان
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