کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4495867 1623817 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Key role of Dkk3 protein in inhibition of cancer cell proliferation: An in silico identification
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Key role of Dkk3 protein in inhibition of cancer cell proliferation: An in silico identification
چکیده انگلیسی


• We model Dkk3 protein.
• Dkk-3 efficiently interacts with Krm, LRP and EGFR.
• Dkk3 plays important role to inhibit cancer cell proliferation.

Dkk3 is a member of Dkk family proteins, regulating Wnt signaling. Dkk3 plays different roles in human and mouse tumors. Dkk3 predominantly act as a tumor suppressor, however several reports revealed that Dkk3 could accelerate cancer cell proliferation. Herein, we aimed at launching an in silico study to determine Dkk3 structure and its interactions with Kremen and LRP as Wnt signaling receptors as well as EGF receptor. Using various softwares a model was built for Dkk3 molecule. Different protein modeling approaches along with model refinement processes were employed to arrive at the final model. To achieve the final complex of Dkk3 with Kremen, LRP and EGFR molecules protein–protein docking servers were employed. Model assessment softwares indicated the high quality of the finally refined Dkk3 3D structure, indicating the accuracy of modeling and refinement process. Our results revealed that Dkk3 is capable of interacting with Kremen, LRP and EGFR with comparable binding energies. Dkk3 efficiently interacts with LRP, Kremen and EGF receptor and may be a promising protein in cancer therapy by blocking Wnt and EGFR downstream signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Theoretical Biology - Volume 393, 21 March 2016, Pages 98–104
نویسندگان
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