کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4501948 | 1320517 | 2011 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Mapping and Comparative Analysis of QTL for Rice Plant Height Based on Different Sample Sizes within a Single Line in RIL Population Mapping and Comparative Analysis of QTL for Rice Plant Height Based on Different Sample Sizes within a Single Line in RIL Population](/preview/png/4501948.png)
To clarify the most appropriate sample size for obtaining phenotypic data for a single line, we investigated the main-effect QTL (M-QTL) of a quantitative trait plant height (ph) in a recombinant inbred line (RIL) population of rice (derived from the cross between Xieqingzao B and Zhonghui 9308) using five individual plants in 2006 and 2009. Twenty-six ph phenotypic datasets from the completely random combinations of 2, 3, 4, and 5 plants in a single line, and five ph phenotypic datasets from five individual plants were used to detect the QTLs. Fifteen M-QTLs were detected by 1 to 31 datasets. Of these, qph7a was detected repeatedly by all the 31 ph datasets in 2006 and explained 11.67% to 23.93% of phenotypic variation; qph3 was detected repeatedly by all the 31 datasets and explained 5.21% to 7.93% and 11.51% to 24.46% of phenotypic variance in 2006 and 2009, respectively. The results indicate that the M-QTL for a quantitative trait could be detected repeatedly by the phenotypic values from 5 individual plants and 26 sets of completely random combinations of phenotypic data within a single line in an RIL population under different environments. The sample size for a single line of the RIL population did not affect the efficiency for identification of stably expressed M-QTLs.
Journal: Rice Science - Volume 18, Issue 4, December 2011, Pages 265-272