کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4502030 | 1320523 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Purification and Analysis of Abscisic Acid-Specifically-Inducible Proteins from Rice Callus Purification and Analysis of Abscisic Acid-Specifically-Inducible Proteins from Rice Callus](/preview/png/4502030.png)
Two ABA-specifically-inducible proteins from rice callus were isolated and purified by precipitation with 65–100% saturated (NH4)2SO4, followed by the DEAE-sepharose, TSK-gel, and two-dimension electrophoresis. Iso-electric points (pI) of the proteins with the same molecular mass (24.5 kD) were 6.1 and 6.9, respectively. The Western blot analysis indicated that the proteins expressed in different tissues were obviously different. The A1 (pI 6.1) protein was only detected in calli treated with ABA and seed embryos (SE). However, the A2 (pI 6.9) protein was found not only in the calli treated with ABA and SE, but also in the white dry callus. Thus it suggested that the two proteins might play some important roles in the processes of seed embryo (or somatic embryo) formation.
Journal: Rice Science - Volume 14, Issue 2, June 2007, Pages 111-117