کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4502154 1320532 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of LOX3 Gene in Alleviating Adverse Effects of Drought and Pathogens in Rice
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
Role of LOX3 Gene in Alleviating Adverse Effects of Drought and Pathogens in Rice
چکیده انگلیسی

Lipoxygenase 3 (LOX3) is a major component of the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under stresses, a plant expression vector containing antisense cDNA of LOX3 was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by the Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. Analyses of embryo LOX3 deletion and semi-quantitative RT-PCR confirmed the antisense suppression of LOX3 gene in transgenic plants. The T2 antisense plants of LOX3 were sensitive to drought stress, rice blast and bacterial blight compared with non-transgenic plants. These results suggest that the LOX3 gene might function in response to stresses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Rice Science - Volume 15, Issue 4, December 2008, Pages 276-282