Novel Germplasm with High Oil Content and Resistance to Aspergillus flavus and Bacterial Wilt Developed from Peanut Recombinant Inbred Lines

The enhancement of resistances to aflatoxin contamination and bacterial wilt is one of the major objectives in peanut (Arachis hypogaea L.) breeding for high oil content. In this study, 117 recombinant inbred lines (RILs) were developed through crossing peanut varieties Yuanza 9102 and Zhonghua 5. Genetic analysis revealed that aflatoxin resistance was controlled by 2 major genes plus additive polygenes and seed oil content controlled by 2 suppressive major genes plus additive polygenes. The RILs showed transgressive segregations in aflatoxin and oil content compared to their parents. Eighteen high oil content lines with resistance to aflatoxin contamination or bacterial wilt were selected from the RILs population. Among them, line J091 exhibited resistance to both diseases. The 18 RILs had genetic diversity based on phenotyping and molecular marker analyses.

摘 要协同提高抗青枯病花生品种的黄曲霉抗性及含油量是中国花生育种的重要目标之一。利用远杂9102×中花5号杂交后代衍生的重组近交系群体(RIL), 通过黄曲霉抗性、青枯病抗性鉴定及含油量测试, 表明黄曲霉抗性受2对连锁并具累加作用主基因+加性多基因控制, 含油量受2对具抑制作用主基因+加性多基因控制, RIL群体的黄曲霉抗性和含油量变异远远超过双亲的差异, 表明它们均具有通过互补产生超亲性状的潜力。获得了抗黄曲霉或抗青枯病的高油后代家系18份, 其中抗黄曲霉兼抗青枯病高油新种质1份(J091)。农艺性状和SSR分析结果表明, 18份后代材料具丰富的遗传多样性, 农艺性状优良, 具有重要育种价值。