Development and Chromosome Mapping of New Wheat EST-SSR Markers and Application for Characterizing Rye Chromosomes Added in Wheat

On the basis of the reported expressed sequence tags (ESTs) that are related to salinity stress and stem in wheat (Triticum aestivum L.), 81 new EST-derived simple sequence repeat (eSSR) markers were developed. Among these markers, 67, 46, 18, and 61 eSSRs produced 124, 72, 26, and 124 stable amplicons in the genomes of wheat, rye (Secale cereals L.), Haynaldia villosa L. Schur., and barley (Hordeum vulgare L.), respectively, indicating their high transferability. Totally, 81 loci amplified by 43 markers were mapped on 18 wheat chromosomes. Eight eSSRs from the 30 markers, which produced specific loci in rye, were mapped on chromosomes 1R, 4R, 5R, and R7 of rye, and 7 eSSRs were located on more than 1 chromosome. The remaining 15 eSSRs might be used to trace chromosomes that are not involved in this study.