Serum Vitamin A Level Measurement in Slaughtered and Live Cattle Using Multispectral Imaging

The optimum wavelength for measuring pupil reflection in order to estimate serum vitamin A level in slaughtered and live cattle was investigated. A multispectrum camera was used to obtain the eye images. The highest coefficient of determination 0.76 between pupil reflection and serum vitamin A level was obtained at 500 nm. Since the photosensitive pigment rhodopsin has a maximum absorption of about 500 nm, the results suggest that 500 nm could be the optimum wavelength for measuring pupil reflection.