Antibacterial activity of Carum copticum extract loaded MnFe2O4 nanoparticles coated with PEGylated chitosan

• Hence new therapeutic approaches for effective treatment are necessary.
• Significant antibacterial effect against gram negative bacteria was developed.
• The obtained nanoparticles exhibited in vitro sustained release profile.

To deal with antibiotic resistance, utilization of natural antibacterial agents such as essential oils and extracts of medicinal plants seems necessary. Carum copticum is a traditional famous spice and food preservative in Indian medical system. C. copticum seeds extract have wealthy antibacterial compounds, hence to enhance stability and antibacterial activity, we loaded CE on PEGylated chitosan-MnFe2O4 NPs, which has significant antibacterial activity especially against gram negative bacteria. The nanoparticle size and crystal structure, respectively demonstrate by XRD and SEM image. CE loading analysis was investigated by FT-IR. VSM was developed to evaluate synthesized NPs magnetic properties. Releasing profile and actual CE loading were determined by UV–vis spectroscopy at (λ = 275 nm). Nanoparticle size was measured approximately 21 nm with spherical and uniform shape. MIC of CE-PEG-chitosan-MnFe2O4 NPs were determined by liquid broth dilution method. Chitosan as biodegradable and compatible natural polymer not only has antibacterial effect, but also improves nanoparticles characteristics. We stabilized NPs with PEG against RES which increases NPs circulation in-vivo.