Trans-resveratrol micronization by SEDS technique

• Application of SEDS (Solution Enhanced Dispersion by Supercritical Fluids) in the micronization of trans-resveratrol.
• It was possible to reduce resveratrol particle size about 167 times with a final value of 220 nm.
• Residual solvent values are within the limits established by the regulations around the world such as Europe, USA and China.

Trans-resveratrol is a polyphenol found in a variety of foods, especially in blackberries, grape seeds, grape skin and in red wine. This compound presents countless human health benefits, including antioxidant, antiplaquetary, antifungal, anti-inflammatory and cardioprotective activities, among others. The aim of this work was to investigate the application of SEDS (Solution Enhanced Dispersion by Supercritical Fluids) technique in the micronization of trans-resveratrol using a 23 Central Composite Design (CCD) with 3 central points so as to assess the influence of compound concentration (4, 12 and 20 mg mL−1), temperature (308, 313 and 318 K) and pressure (8, 10 and 12 MPa) on the particle size (Dp) produced. It was noted that the mean particle size obtained at 20 mg mL−1 resveratrol concentration, 8 MPa and 308 K was 0.22 μm, a significant reduction compared with the original value of 36.65 μm. In the majority/in most of the experiments spherical particles were obtained. At 95% confidence level temperature showed a positive effect while operating pressure and the interaction pressure × temperature showed a significant, negative effect, on the particle size. Analysis of micronized material proved that it is in compliance with pharmaceutical human consumption standards.