کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4512950 1624840 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical composition and antiproliferative activity of Acalypha californica
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Chemical composition and antiproliferative activity of Acalypha californica
چکیده انگلیسی


• The antiproliferative activity of methanol extract of Acalyphacalifornica and its fractions of solvents were evaluated.
• The chemical characterization of the active fractions allowed the identification of β-sitosterol and stigmasterol, some phenolic acids, proanthocyanidins and flavonoids.
• A. californica showed against the M12.Ak.C3.F6, RAW 264.7, HeLa and L929 cell lines.
• The methanol extract and hexane fraction reduces mitochondrial membrane potential significantly and activates caspases 3, 8 and 9.
• The extracts induce apoptosis via activation of caspases.

Acalypha californica Benth., is a plant in the northwestern region from Mexico, commonly known as “cancer herb” and used in traditional medicine for treating cancer. In the present study we have investigated the antiproliferative activity of methanolic extract of A. californica and its fractions in cancer cell lines and phytochemical analysis and mechanism of apoptosis of the fractions with antiproliferative activity. The antiproliferative activity of methanol extract and its fractions of solvents were evaluated by MTT assay against the M12.Ak.C3.F6, RAW 264.7, HeLa and L929 cell lines. Active fractions were fractionated by molecular exclusion chromatography, HPLC and MPLC. The identification of compounds was performed by NMR and FIA-ESI-IT-MS/MS analysis. Apoptotic mechanism was analyzed by flow cytometry, determining the reduction in the mitochondrial membrane potential (JC-1) and the activity of caspases 3, 8 and 9. Cell viability assays showed that the hexane fraction of the methanol extract of the plant has significant effects against cancer lines RAW 264.7 (IC50 = 52.08 ± 1.06 μg/mL) and HeLa (IC50 = 46.77 ± 1.09 μg/mL), the residual fraction showed a selective effect on cell lines M12.Ak.C3.F6 (IC50 = 59.90 ± 1.05 μg/mL), RAW 264.7 (IC50 = 58.93 ± 1.26 μg/mL) and HeLa (IC50 = 50.11 ± 1.135 μg/mL) compared to the control cell line L929 (IC50 = 100.00 ± 1.09 μg/mL). The chemical characterization of the active fractions allowed the identification of β-sitosterol and stigmasterol in hexane fraction and some phenolic acids, proanthocyanidins and flavonoids in the residual fraction. The methanol extract and hexane fraction reduces mitochondrial membrane potential significantly and activates caspases 3, 8 and 9. Because of the antiproliferative activity observed, our results provide a rational basis for the use of extracts of A. californica in treating various types of cancer in traditional medicine from Mexico. The extracts induce apoptosis via activation of caspases.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 69, July 2015, Pages 48–54
نویسندگان
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