Large scale extraction of the fruits of Garcinia indica for the isolation of new and known polyisoprenylated benzophenone derivatives

Large scale extraction of the fruits of Garcinia indica was conducted with water extracted hydroxyl citric acid. The marc left after water extraction was extracted exhaustively with methanol and concentrated methanol. The concentrated methanol extract was adsorbed with celite and dried. The dried celite containing the methanol extract was successively extracted with hexane, chloroform and ethyl acetate to get hexane, chloroform and ethyl acetate extract respectively. Column chromatographic separation of hexane extract afforded two new molecules, one polyisoprenylated benzophenone and a new acylphloroglucinol derivative and two known molecule garcinol and isogarcinol. Likewise, chromatographic separation of the chloroform and ethyl acetate extract also yielded extra quantity of garcinol and isogarcinol respectively. Garcinol was isolated as a major compound among all the four molecules isolated from the fruits G. indica.

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► Upscaling of garcinol and isogarcinol.
► Isolation and structure determination of two new compounds from the fruits of Garcinia indica.
► Solid matrix partitioning technology used for large scale isolation.