Pilot scale processing technology for extraction of Cliv-92: A combination of three coumarinolignoids cleomiscosins A, B and C from Cleome viscosa

The paper reports an improved pilot scale process technology for the extraction of Cliv-92: a combination of three hepatoprotective coumarinolignoids, cleomiscosins A, B and C from the seeds of Cleome viscosa. The method involved the extraction of the defatted seeds with methanol, which on concentration gave a viscous residue. Trituration of the residue with methanol and keeping it at a low temperature resulted in the precipitation of Cliv-92 which was separated by filtration. Almost half of total yield of Cliv-92 was obtained from the methanolic extract simply by the trituration method. The filtrate obtained was concentrated and adsorbed on celite. The adsorbed material was then partitioned with toluene, ethyl acetate and methanol successively. The fractions obtained from partitioning methods were concentrated and Cliv-92 was isolated from the above fractions by passing through a bed of silica gel. The novelty of the improved process included application of an extrusion device to expel out the fixed oil before extraction, adopting trituration technique to effectively crystallize the coumarinolignoids and using solid-matrix partitioning technique thereby avoiding the use of water for partitioning to isolate the coumarinolignoids. The yield of Cliv-92 obtained was 1.13% carried out at a scale of 100 kg/batch level which was about 10 times higher in comparison to previous bench scale process.