کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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4521825 | 1625197 | 2007 | 8 صفحه PDF | دانلود رایگان |
In order to provide an insight into molecular basis of cross-fertilized kernel advantage and heterosis, we performed screening of differentially expressed genes between reciprocal cross-fertilized kernels and their parents at 2, 6, and 12 days after pollination (DAP) by using differential display technique. Seventeen differentially expressed cDNAs were verified by reverse-northern blot. Sequence analysis and database search revealed that differentially expressed genes between reciprocal cross-fertilized kernels and their parents included genes involved in metabolism, signal transduction, transcription factor and so on. In silico expression analysis of the 9 differential expressed genes in crown, flower, leave, root, stem, inflorescence and seed tissues, and indicated that they are expressed in various tissue-specific patterns. These results indicated that diverse pathways may involve in wheat heterosis formation.
Journal: South African Journal of Botany - Volume 73, Issue 4, November 2007, Pages 522–529