کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4531276 | 1324766 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acute toxicity profile of cadmium revealed by proteomics in brain tissue of Paralichthys olivaceus: Potential role of transferrin in cadmium toxicity
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کلمات کلیدی
PMF2D-PAGEParalichthys olivaceusCBCESI-MSAASTransferrinnuclear magnetic resonance - رزونانس مغناطیسی هستهایPeptide mass fingerprinting - اثر انگشت پپتیدهElectrospray Ionization Mass Spectrometry - اسپکترومتر جرم یونیزاسیون ElectrosprayscAT - اسکاتtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیBrain tissue - بافت مغزNMR - تشدید مغناطیسی هستهای Contamination level - سطح آلودگیMass spectrometry - طیف سنجی جرمیmatrix-assisted laser desorption/ionization mass spectrometry - طیف سنجی جرمی لیزری جذب / یونیزاسیون لیزر ماتریسMALDI-TOF MS - مالدی توف MSMetallothionein - متالوتونیئینProteomics - پروتئومیکسDifferential proteins - پروتئین های دیفرانسیل
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
An analytical approach using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) separated proteins from the brain tissue of the fish Paralichthys olivaceus. Approximately 600 protein spots were detected from the brain sample when applying 600 μg protein to a 2D-PAGE gel in the pH range 3.5-10.0. Compared to a control sample, significant changes of 24 protein spots were observed in the fish tissue exposed to acute toxicity of seawater cadmium (SCAT) at 10 ppm for 24 h. Among these spots, nine were down-regulated, nine were up-regulated, two showed high expression, and four showed low expression. The collected spots were identified by peptide mass fingerprinting (PMF) and database search, and they were further classified by LOCtree, a hierarchical system of support vector machines which predict their sub-cellular localization. The amount of transferrin expression in brain cells decreased linearly with the increase of SCAT concentration in seawater. Among the 24 proteins identified on a 2D-PAGE gel, 9 demonstrated a synchronous response to acute cadmium, suggesting that they might represent a biomarker profile. Based on their variable levels and trends on the 2D-PAGE gel this protein (likely to be transferrin) suggesting they might be utilized as biomarkers to investigate cadmium pollution levels in seawater and halobios survival, as well as to evaluate the degree of risk of human fatalities. The results indicate that the application of multiple biomarkers has an advantage over a single biomarker for monitoring levels of environmental contamination.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquatic Toxicology - Volume 78, Issue 2, 15 June 2006, Pages 127-135
Journal: Aquatic Toxicology - Volume 78, Issue 2, 15 June 2006, Pages 127-135
نویسندگان
Jin-Yong Zhu, He-Qing Huang, Xiao-Dong Bao, Qing-Mei Lin, Zongwei Cai,