کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4545440 | 1626945 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cyclin B gene and its cell cycle-dependent differential expression in the toxic dinoflagellate Alexandrium fundyense Atama Group I/Clade I
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کلمات کلیدی
CDKRT-qPCRMitotic cyclinToxic dinoflagellate - Dinoflagellate سمیBLAST, basic local alignment search tool - ابزار پایهای برای جستجوی برهم نهیهای موضعی، بلاستEST - استBlast - انفجارGene expression - بیان ژنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدExpressed Sequence Tag - تکرار اظهار نظرquantitative reverse transcription PCR - رونویسی معکوس PCR کمیharmful algal blooms - شکوفه های جلبک مضرRace - مسابقهCell cycle - چرخه سلولیcyclin-dependent kinase - کییناز وابسته به سیکلین
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Cell cycle regulation is the intrinsic determinant of phytoplankton population dynamics since cell division in unicellular organisms directly leads to population growth. Central in the regulatory engine are cyclin/CDK complexes. Cyclin B, which is highly accumulated during the G2 and M phases (often combined as G2M) of the cell cycle, can be a growth rate marker useful for harmful algal bloom research. In this study, we isolated the full-length cDNA of a cyclin B-like gene from the toxic dinoflagellate Alexandrium fundyense Atama Group I/Clade I (Afcyc, 1669Â bp). The deduced protein sequence (AFCYC, 455 amino acids) is closest (68% similarity) to, and phylogenetically clustered with the mitotic cyclin documented in Lingulodinium polyedrum. This sequence contains two cyclin-specific domains, the destruction box and the cyclin B signature motif, verifying that it is cyclin B. Using quantitative reverse transcription PCR (RT-qPCR), we characterized the dynamics of Afcyc expression throughout the cell cycle. Afcyc transcript abundance was over 6-fold higher in the G2M phase than in other cell cycle phases, and showed a positive correlation with the percentage of cells in the G2M phase. Our results suggest (1) the mitotic cyclin-based cell cycle regulation is likely conserved in gonyaulacoid dinoflagellates; (2) Afcyc is transcriptionally regulated, and (3) Afcyc is a candidate growth marker for monitoring the growth rates of A. fundyense Atama Group I/Clade I.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Harmful Algae - Volume 26, June 2013, Pages 71-79
Journal: Harmful Algae - Volume 26, June 2013, Pages 71-79
نویسندگان
Yunyun Zhuang, Huan Zhang, Senjie Lin,