کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4561746 1330688 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Epidemiology and antibiotic resistance of Salmonella enterica Serovar Kentucky isolates from Tunisia: The new emergent multi-drug resistant serotype
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش تغذیه
پیش نمایش صفحه اول مقاله
Epidemiology and antibiotic resistance of Salmonella enterica Serovar Kentucky isolates from Tunisia: The new emergent multi-drug resistant serotype
چکیده انگلیسی

Since 2002, the emergence of multidrug resistant Salmonella enterica serovar Kentucky (S. Kentucky) and the associated salmonellosis with treatment failure were declared in different parts of the world and were in most of the case contracted during travels to Northeast and Eastern Africa. In the present work, we reported an epidemiological study of S. Kentucky isolated from different environmental and clinical origins in Tunisia, using Pulsed-field gel electrophoresis (PFGE); Enterobacterial Repetitive Intergenic Consensus (ERIC-2) fingerprinting; Plasmid profiling; and antibiotic resistance profiles. ERIC-2 fingerprinting allowed the differentiation of 14 different patterns versus only 4 pulsotypes. Besides, a high proportion of strains were found to be nontypeable by XbaI-PFGE and/or by plasmid profiling (plasmid-free strains). The antibiotic resistance was mainly detected against streptomycin (80.7%), sulfonamides (42.1%) and tetracycline (15.7%). Furthermore, two avian strains were shown to be resistant to trimethoprim–sulfamethoxazole and three clinical strains have demonstrated multidrug-resistant phenotypes (against 5 to 10 antibiotics) and all of them exhibited resistance against nalidixic acid, ciprofloxacin, ofloxacin and tetracycline.ERIC-2 PCR was found to be the most discriminative. However, combination of the three typing methods offer a better mean for differentiating S. Kentucky isolates, monitoring the multiresistant types and determination of their origin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Food Research International - Volume 45, Issue 2, March 2012, Pages 925–930
نویسندگان
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