کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4565840 1331085 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of Powdery Mildew Resistance in Wild Melon MLO Mutants
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک جنگلداری
پیش نمایش صفحه اول مقاله
Analysis of Powdery Mildew Resistance in Wild Melon MLO Mutants
چکیده انگلیسی

Wild species have a potential value in crop breeding. Explore MLO gene which related with powdery mildew natural resistance is very important for improving the quality of melon. Resistance to powdery mildew was examined in cultivar and wild species by leaf inoculation. The wild germplasms showed resistance to powdery mildew Race1. Cloning and sequence analysis of the CmMLO2 gene identified an 85 bp difference between the wild and cultivated species. The CmMLO2 gene was expressed in the wild germplasm after fluorescence-labeled Agrobacterium-mediated transformation. A positive transgenic plant showed successful invasion by powdery mildew Race1. These results suggested that the wild species might have failed to encode the MLO protein, thereby resulting in the MLO-negative regulation of powdery mildew, which in turn resulted in the broad-spectrum resistance of the wild species to powdery mildew.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Horticultural Plant Journal - Volume 1, Issue 3, November 2015, Pages 165–171
نویسندگان
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