Molecular characterization of thaumatin family genes related to stresses in Brassica rapa

Biotic and abiotic stresses are a major concern for Brassica, which is a very important vegetable group worldwide. Enhancement of resistance by exploiting stress resistance related genes offers the most efficient approach to addressing this issue. Among the stress resistance related genes, thaumatin-like proteins (TLPs) play a vital role in enhancement of resistance against stresses. In this study, we identified 20 TLPs from our full-length cDNA library of Brassica rapa cv. Osome and the Brassica database. The sequences were then analyzed and compared with other published pathogenesis related TLPs and found a high degree of homology with them. In addition, these genes showed organ specific expression, four genes responded after Pectobacterium carotovorum subsp. carotovorum infection and seven genes showed responses to cold, salt, drought and abscisic acid (ABA) stress treatments in chinese cabbage. Taken together these data revealed that these genes may be involved in resistance against stresses for Brassica.

► We identify 20 thaumatin-like genes and analyze the sequences.
► Genes show high homology with other pathogenesis-related thaumatin-like genes.
► These genes show organ specific expression and responses after treatment of stresses.
► These genes may involve in resistance to biotic and abiotic stresses for Brassica.