Growth, photosynthesis and antioxidant metabolism in mustard (Brassica juncea L.) cultivars differing in ATP-sulfurylase activity under salinity stress

Salinity is one of the major environmental factors limiting crop productivity. The effect of increasing salinity levels (0, 50, 100 mM NaCl) on growth, photosynthetic traits, leaf water potential, oxidative stress, enzymatic and non-enzymatic antioxidants was studied in Pusa Jai Kisan and SS2 cultivars of mustard (Brassica juncea L. Czern & Coss.) differing in ATP-sulfurylase activity at 30 days after sowing (DAS). The cultivar SS2 (low ATP-sulfurylase activity) accumulated higher content of Na+ and Cl− in leaf than root. SS2 also showed greater content of thiobarbituric acid reactive substances (TBARS) and H2O2 and higher decrease in growth, photosynthetic traits and leaf water potential than Pusa Jai Kisan with increasing salinity levels. Contrarily, Pusa Jai Kisan (high ATP-sulfurylase activity) exhibited higher Na+ and Cl− content in root than leaf, lower TBARS and H2O2 content and higher activity of catalase, ascorbate peroxidase and glutathione reductase. However, the activity of superoxide dismutase was greater in SS2 than Pusa Jai Kisan. Higher activity of ATP-sulfurylase in Pusa Jai Kisan resulted in increased content of glutathione, a reduced form of inorganic sulfur and an essential component of cellular antioxidant defense system. The lesser decrease in growth and photosynthesis in Pusa Jai Kisan was the result of lesser Na+ and Cl− in leaf, higher turgidity and increased activity of antioxidant enzymes and glutathione content.