کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569223 | 1331328 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Potential regulation of apple in vitro shoot growth via modulation of cellular polyamine contents
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Potential regulation of apple in vitro shoot growth via modulation of cellular polyamine contents Potential regulation of apple in vitro shoot growth via modulation of cellular polyamine contents](/preview/png/4569223.png)
چکیده انگلیسی
In the present research attempts were made to elucidate effects of d-arginine (d-arg), a potential inhibitor of arginine decarboxylase (ADC) involved in polyamine biosynthesis, on growth and polyamine biosynthesis of apple [Malus sylvestris (L.) Mill. var. domestica (Borkh.) Mansf.] in vitro shoots. Growth of the shoots cultured on proliferation medium added with 1 or 5Â mM d-arg was inhibited over a 3-week treatment, with 5Â mM being more effective. Activity of ADC was slightly or remarkably inhibited by 1 and 5Â mM d-arg, respectively, in line with change of free putrescine (Put) content in the treated shoots. Activity of ornithine decarboxylase (ODC) was only repressed by 5Â mM d-arg. Application of exogenous Put at 1Â mM slightly recovered the growth of impaired apple shoots, while exogenous Put at 5Â mM could resume the growth to a level similar with the control. Addition of exogenous Put led to notable increase in endogenous free Put, coupled with corresponding recovery of ADC and ODC activities. Transcriptional levels of several key genes involved in polyamine biosynthesis were more or less repressed by 5Â mM d-arg relative to the untreated shoots, which were otherwise up-regulated by exogenously applied Put. The data presented herein showed that it is possible to regulate apple shoot growth via modulation of cellular polyamine contents.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 119, Issue 4, 17 February 2009, Pages 423-429
Journal: Scientia Horticulturae - Volume 119, Issue 4, 17 February 2009, Pages 423-429
نویسندگان
Ji-Hong Liu, Kazuyoshi Nada, Toshihito Kurosawa, Yusuke Ban, Takaya Moriguchi,