کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4569245 | 1331329 | 2009 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش باغداری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm Isoenzyme and microsatellite analysis of Vitis vinifera L. varieties from the Hungarian grape germplasm](/preview/png/4569245.png)
چکیده انگلیسی
The aim of our work was to investigate the genetic diversity of grapevine with biochemical and molecular markers (isoenzyme and SSR). The isoenzyme patterns of 4 enzyme systems (catechol-oxidase, glutamate-oxalacetate-transaminase, acid phosphatase and peroxidase) and the microsatellite profile in 6 loci (VVS2, VVS16, VVMD7, VMC4A1, VMC4G6, VrZag79) of 48 grapevine varieties were analysed. The results with CO, GOT, AcP and PER enzymes were reproducible and the zymograms obtained from the woody stems were independent from the time of sampling during the dormant period of the grape. Based on the isoenzyme patterns of these 4 enzymes most of the investigated varieties (40/48) were identified. A correlation was found between the isoenzyme patterns and the classification to convarietas of the varieties. It was established, that while the varieties of the convarietas pontica differed from those of the convarietas orientalis and occidentalis, the two latter groups could have not been differentiated from each other. Based on the SSR (simple sequence repeat) analyses 46 of the 48 investigated varieties were identified. Even 'Pinot blanc' and 'Pinot gris' cultivars belonging to the same conculta (Pinot) could be differentiated in their VMC4A1 locus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 120, Issue 2, 2 April 2009, Pages 213-221
Journal: Scientia Horticulturae - Volume 120, Issue 2, 2 April 2009, Pages 213-221
نویسندگان
G. Jahnke, J. Májer, A. Lakatos, J. Györffyné Molnár, E. Deák, Ã. Stefanovits-Bányai, P. Varga,