Peptone stimulates in vitro shoot and root regeneration of avocado (Persea americana Mill.)

The effect of peptone on in vitro regeneration of avocado's shoots and roots from juvenile or mature stem sections was studied. In this study, both mature and juvenile explants were used to study the effect of peptone on shoot regeneration. Explants were necrotized on Murashige and Skoog (MS) medium without or with plant growth regulators. On the MS medium supplemented with both peptone and benzyladenine or dichlorophenoxyacetic acid, explants survived but significantly delayed in shoot regeneration. Addition of peptone alone into the medium with an optimal amount of 2.0% (w/v) induced shoot formation. Shoot formation also occurred in nodal sections from mature plant but the regeneration rate remained low. All juvenile explant-derived shoots formed root on the medium supplemented with peptone and naphthaleneacetic acid.