کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4569595 1331346 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش باغداری
پیش نمایش صفحه اول مقاله
Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local
چکیده انگلیسی

A protocol for plant regeneration by somatic embryogenesis was developed in guava cv. Banarasi local by using immature zygotic embryo explants. Best induction of somatic embryogenesis was achieved from 10-week-old zygotic embryos on MS medium supplemented with 2,4-d (4.52 μM) and 5% sucrose. Maximum number of somatic embryos was produced when zygotic embryo explants were transferred to growth regulator free full strength MS basal medium after 8 days treatment with 2,4-d. Full strength MS basal medium containing 5% sucrose was most favorable for maturation of somatic embryos. Highest frequency of conversion and normal plantlet production were recorded from elongated torpedo stages of somatic embryos on half strength MS medium containing 3% sucrose. Over 90% of rooted shoots survived acclimatization.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Scientia Horticulturae - Volume 113, Issue 2, 26 June 2007, Pages 129–133
نویسندگان
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