Drought effect on electrophoretic protein pattern of Anoectochilus formosanus

Drought effect on two dimensional iso-electric focusing/sodium dodecyl sulfate-poly acrylamide gel electrophoretic (2-D IEF/SDS-PAGE) protein pattern was studied in Anoectochilus formosanus leaves under ex vitro condition. Spectrophotometery analysis revealed that under drought condition protein content decreased significantly. Similarly, 2-D IEF/SDS-PAGE and PDQuest software analysis indicated that out of 17 protein spots, expression of 15 protein spots were quantitatively down-regulated and 2 spots were up-regulated. Moreover, 11 protein spots were qualitatively up-regulated while 6 were down-regulated.