کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4750563 1642530 2011 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A direct comparison of three palynological preparation techniques
موضوعات مرتبط
مهندسی و علوم پایه علوم زمین و سیارات فسیل شناسی
پیش نمایش صفحه اول مقاله
A direct comparison of three palynological preparation techniques
چکیده انگلیسی

Two samples of palynomorph-rich Upper Jurassic (Lower Oxfordian) mudstone from western Scotland were quantitatively prepared using the traditional hydrochloric and hydrofluoric acid based palynological preparation technique and two non-acid procedures. The latter are protocols using sodium hexametaphosphate [(NaPO3)6] and hydrogen peroxide (H2O2). These non-acid techniques have previously been validated only in terms of the absolute numbers of palynomorphs extracted. By contrast, this study aimed to assess the numbers of palynomorphs extracted in terms of absolute numbers of the individual taxa present to test for any taxonomic biases. The (NaPO3)6 method proved around 50% as efficient as acid digestion in terms of absolute numbers of palynomorphs extracted. It produced clean residues, which are eminently suitable for most palynological studies. The majority of the taxa present were recovered in representative relative proportions, and no taxonomic biases were noted. The absolute numbers of most taxa decrease in a stepwise fashion from acid digestion via the (NaPO3)6 procedure to the H2O2 method. However, the concentrations of bisaccate pollen were apparently relatively unaffected by the three methods used. Similarly, the Meiourogonyaulax caytonensis group appears to be unusually resistant to oxidation damage by H2O2. It is considered that the (NaPO3)6 preparation method is an eminently viable alternative to acid digestion, especially in remote operational settings such as rigsites. The H2O2 technique proved to be significantly less effective, at approximately 10% of the extraction level of acid digestion which appears to be largely due to oxidation. Hydrogen peroxide is an aggressive oxidant. Therefore the (NaPO3)6 technique is deemed to be both safer and more effective than the H2O2 method.


► Acid digestion was compared to two ‘non-acid’ palynological preparation techniques.
► Two Jurassic palynomorph-rich samples were tested.
► Concentrations of taxa were analysed for method-specific biases.
► No such biases were identified.
► The sodium hexametaphosphate technique is an effective alternative to acid digestion.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Review of Palaeobotany and Palynology - Volume 167, Issues 3–4, October 2011, Pages 212–221
نویسندگان
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