کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4756616 | 1419013 | 2017 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Synthesis of (S)-3-chloro-1-phenylpropanol by permeabilized recombinant Escherichia coli harboring Saccharomyces cerevisiae YOL151W reductase in 2-methyltetrahydrofuran cosolvent system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
کاتالیزور
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
2-methyltetrahydrofuran, an ecofriendly substitute for traditional organic solvents, was employed to construct cosolvent system for synthesis of (S)-3-chloro-1-phenylpropanol by permeabilized recombinant Escherichia coli containing the YOL151W reductase and Escherichia coli containing d-glucose dehydrogenase to regenerate NADPH. By optimization, 30Â mM 3-chloro-1-phenyl-1-propanone could be efficiently converted to (S)-3-chloro-1-phenylpropanol with a yield of 98.6% and enantiomeric excess of more than 99.9% in 3% (v/v) of 2-methyltetrahydrofuran, 2% (v/v) of Triton X-100 and Tris-HCl buffer coslovent system. The application of 2-methyltetrahydrofuran has proved to be a promising cosolvent for asymmetric reduction of water-insoluble prochiral ketones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Catalysis Communications - Volume 97, 5 July 2017, Pages 56-59
Journal: Catalysis Communications - Volume 97, 5 July 2017, Pages 56-59
نویسندگان
Yanjun Tian, Xiaoqiang Ma, Meng Yang, Dongzhi Wei, Erzheng Su,