Identification and quantification of synthetic cannabinoids in 'spice-like' herbal mixtures: Update of the German situation in early 2017

- Identification and quantification of MDMB-CHMICA, 5F-Cumyl-P7AICA and Cumyl-PeGACLONE in herbal smoking mixtures.
- Full physico-chemical characterization of the new 5F-Cumyl-P7AICA and Cumyl-PeGACLONE.
- Global minima calculation comparison for Cumyl-PeGACLONE and JWH-018.
- GC-MS quantification of all synthetic cannabinoids using JWH-018 as internal standard.

In February 2017, eleven “Spice-like” products (14 individual packages including replicates) from German language internet shops were analyzed. In total, three different synthetic cannabinoids (SCs) were identified by gas-chromatography-mass spectrometry (GC-MS), namely MDMB-CHMICA and two, so far only partially described compounds, 5F-Cumyl-P7AICA and Cumyl-PeGACLONE. All analyzed products contained only one synthetic cannabinoid as active ingredient. 5F-Cumyl-P7AICA and Cumyl-PeGACLONE were subject to an in-depth characterization by nuclear magnetic resonance spectroscopy (NMR), electron ionization mass spectrometry (EI-MS), electrospray ionization tandem mass spectrometry (ESI-MS/MS), infrared and ultraviolet-visible spectroscopy (IR and UV/Vis). Cumyl-PeGACLONE shows a rather unexpected structure compared to conventional SCs of the past. Hence a global minima calculation was conducted to demonstrate structural similarity of Cumyl-PeGACLONE to JWH-018, a classical SC.In addition, all SCs were quantified by a GC-MS method using JWH-018 as internal standard and corresponding response factors. While MDMB-CHMICA was detected in six out of 14 tested products (ranging from 6 to 20 mg/g; average 10 mg/g), 5F-Cumyl-P7AICA and Cumyl-PeGACLONE were detected in three (109-153 mg/g; average 131 mg/g) and five products (15-74 mg/g; average 39 mg/g), respectively.

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