کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
4760268 1421973 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nanoscale characterization of forensically relevant epithelial cells and surface associated extracellular DNA
ترجمه فارسی عنوان
مشخصه نانومقیاس سلول های اپیتلیال و دیابتی های خارج سلولی مرتبط با آن
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Atomic force microscopy provides a novel morphological and physico-chemical perspective to analyze epithelial cell samples in forensic investigations. As a nanoscale, single cell tool, it allows the investigation of scarce samples in a non-destructive fashion. Using chemical force spectroscopy, it permits the identification of specific functional groups or surface molecules. Of specific interest is the presence of extracellular DNA (eDNA) on the surface of epithelial cells that line the exterior skin and interior cavities of human bodies, and can transfer onto surfaces through contact with skin and saliva. To date, this eDNA has only been measured a bulk level. Here, using nanoscale imaging, we first describe the unique differences between keratinized epithelial cells and non-keratinized buccal cells. Then via a force mapping technique, we show how eDNA can be spatially located and quantified on the cell surface. Our results suggest that presence and relative quantity of surface-associated, extracellular DNA signatures can be analyzed on individual epithelial cells from different tissue sources, providing a new tool in the forensic analysis of touch samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Forensic Science International - Volume 277, August 2017, Pages 252-258
نویسندگان
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