کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4766089 | 1423896 | 2017 | 40 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A lysosomal probe for monitoring of pH in living cells and ovarian tumour
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
Intracellular pH is important in regulating various cellular behaviour and pathological conditions. We synthesised a rhodamine-based lysosomal pH probe (hereafter referred to as RD) with a pKa of 4.10. The probe exhibited favourable “on-off” reversibility between pH 3.25 and 7.20 for at least five cycles. RD can be employed to distinguish cancer cells from normal cells on the basis of different fluorescence response. RD performed well in the detection of lysosomal pH induced by artesunate, chloroquine and dexamethasone in MCF-7Â cells. The intense fluorescence of RD in ovarian tumour indicated the higher acidity in tumour tissues than that in normal tissues. Furthermore, RD worked well in the imaging of excised mouse stomach and living mice under acidic stimulation. These data demonstrate the applicability of RD in monitoring pH in complex biological systems.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 139, April 2017, Pages 318-325
Journal: Dyes and Pigments - Volume 139, April 2017, Pages 318-325
نویسندگان
Bo Li, Guang bo Ge, Lanlan Wen, Ye Yuan, Ran Zhang, Xiaojun Peng, Fengyu Liu, Shiguo Sun,