| کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
|---|---|---|---|---|
| 4910981 | 1427899 | 2016 | 4 صفحه PDF | دانلود رایگان |
کلمات کلیدی
1- مقدمه
2. مواد و روش‌ها
2. 1 CMV ایزوله
2. 2 مراحل پادتن
2. 3 ایجاد منحنی استاندارد
2. 4 مطالعه واکنش‌پذیری متقابل
3. نتایج و بحث
شکل 1: منحنی استاندارد CMV با استفاده از روش ساندویچی ELISA ایجاد شد (حد تشخیص = mg/ml 1/0)
شکل 2: عملکرد حسگر ایمنی برای واکنش‌پذیری متقابل CMV و تایید اعتبار آن با روش‌های بهینه‌سازی‌شده ELISA و کیت ELISA تجاری
4. نتیجه‌گیری
Cucumber mosaic virus (CMV) causes major losses to agricultural and horticultural crops around the world. Hence, a rapid assay for the detection of CMV which can be employed in both laboratory and field is essential. A portable electrochemical immunosensor system for the detection of CMV, based on immobilized CMV specific antibodies conjugated with gold nanoparticle was developed for this purpose. The conjugated antibodies were added with polymer and deposited onto carbon screen printed working electrodes. Optimization of the modified surface immunosensor was performed using sandwich immunoassay format (ELISA). The initial ELISA result for the standard curve development showed a limit of detection down to 0.1mg/mL. Subsequently, the immunosensor was tested for cross reactivity with other plant pathogens. The performance of the electrochemical immunosensor revealed that it has a high selectivity in sample matrix with other organism. This immunosensor provides a promising technology for simple and sensitive detection system that is essential in rapid detection of plant pathogens.
Journal: Procedia Chemistry - Volume 20, 2016, Pages 25-28