Acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) as a novel promiscuous biocatalyst for aldol addition

• ST0779 identified as novel promising promiscuous biocatalyst for aldol addition
• Turnover number kcat of ST0779 at 55 °C is 7.78-fold higher than that of PPL at 37 °C.
• The catalytic efficiency kcat/Km of ST0779 adds up to 140 times higher than PPL.
• Demonstrated the potential to discover novel promiscuous enzymes from the thermophiles

This work, for the first time, demonstrated the catalytic promiscuity of an acyl-peptide releasing enzyme from Sulfolobus tokodaii (ST0779) for aldol addition reaction, which shows accelerated activity at elevated temperature. The turnover number kcat (s− 1) of this thermostable enzyme at 55 °C is 7.78-fold higher than that of porcine pancreatic lipase (PPL) at its optimum temperature of 37 °C, which is one of the best reported enzymes for promiscuous catalysis of aldol reaction; and the molecular catalytic efficiency kcat/Km (M− 1 s− 1) adds up to 140 times higher than PPL.

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