کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4998545 | 1460395 | 2017 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of polyhydroxybutyrate biosynthesis in Bacillus subtilis with combination of PHB-associated genes derived from Ralstonia eutropha and Bacillus megaterium
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
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چکیده انگلیسی
Polyhydroxybutyrate (PHB) is a biodegradable polymer synthesized by bacteria for carbon storage in response to environmental stress. B. subtilis is generally recognized as a safe Gram-positive bacterium and is widely used for industrial-scale production of proteins and chemicals. Owing to lack of toxic lipopolysaccharides, B. subtilis is a superior alternative to the Gram-negative bacteria presently used for PHB production. In this study, we introduced PHB synthesis genes from R. eutropha and B. megaterium into B. subtilis for PHB production. The highest yield of PHB was achieved by coexpression of phaA from R. eutropha and phaR, phaB, and phaC from B. megaterium (up to 13.02% ± 1.67% of total cell dry weight in LB medium containing 1% glucose). The results indicate that B. subtilis is potentially used as a safe vehicle for producing PHB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 79, October 2017, Pages 110-115
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 79, October 2017, Pages 110-115
نویسندگان
Yong-Yang Lin, Po Ting Chen,