کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4998551 | 1460395 | 2017 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Single step purification of bromelain from Ananas comosus pulp using a polymer/salt aqueous biphasic system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
تکنولوژی و شیمی فرآیندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Bromelain is an important industrial proteolytic enzyme due to the wide application in food, cosmetic and therapeutic industries. There is a great demand in developing a cost-efficient method to recover bromelain from plant sources. Low interfacial tension and biocompatible aqueous biphasic system (ABS) is widely applied in various separation processes of bioproducts. Thus, recovery of bromelain from Ananas comosus (pineapple) pulp using polymer/salt ABS was attempted in this study. The ABS operating factors including polyethylene glycol (PEG) molecular weight, phase composition, pH, centrifugal force and additional of neutral salt were studied. The optimal condition for ABS recovery of bromelain from crude extract was achieved with ABS consisting of 17% (w/w) PEG 4000 and 14% (w/w) phosphate salt; centrifugal force of 6000 Ã g; pH of 8.0 and at 25°C. Bromelain exhibited a high specific activity (SA) of 2.7 U and purification factor (PF) of 16.3 in the top phase of PEG 4000/phosphate ABS with a yield (YT) of 55.6%. The findings demonstrated that ABS is a potential one-step operation in replacement to the existing methods in recovery of bromelain from plant sources.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 79, October 2017, Pages 158-162
Journal: Journal of the Taiwan Institute of Chemical Engineers - Volume 79, October 2017, Pages 158-162
نویسندگان
Wen-Chi Wu, Hui Suan Ng, I-Ming Sun, John Chi-Wei Lan,