Novel method for cell penetration based on MEMS technology with failure and impacting characterization of mouse ooctye membrane

Several methods have been investigated to analyze the penetration into oocyte membrane. Among them, micro-electromechanical system technologies have substantial potentials for Intracytoplasmic sperm injection (ICSI) or other penetration applications. In this paper, a novel method has been proposed for penetration into the bio-membrane by arrow and bow concept utilizing a designed MEMS actuator device. Thus, studying the mechanical properties of oocyte membrane is of great importance in this study. In this regard, in order to have a better understanding of the amount of the required force for penetration, failure stress and distribution of stress in contact region would be needed. Here, in the first step, the existing models are reviewed and their shortcomings for analyzing the contact region are discussed. Then, the ICSI process is simulated with finite element method (FEM) in ANSYS-APDL software. Finally by neglecting the plastic deformation of the membrane, its failure stress, Neo-Hookean and Mooney-Rivlin parameters are obtained. Eventually, the required needle velocity for penetration and its related effects after impact has been explored by ANSYS LS-DYNA software.