Blood fluorescence polarization characteristics of saturated fatty acid biological effects

The fluorescence spectra of normal and lard diet intake whole blood solution induced by a laser with an excitation wavelength of 407 nm, and the polarization fluorescence spectra at angles of 0°, 35°, 55° and 90° were studied experimentally. The polarization state was tested by fluorescence intensity Stokes vector parameters. Through Mueller matrix calculations, we discuss the polarization degrees of whole blood solution during different time, the effects on fluorescence parameters of whole blood solution after lard diet intake. The results show that both the main fluorescence peaks of normal and lard diet intake whole blood solution locate at about 612 nm. The polarization degree of the whole blood solution after lard diet intake fluctuates greatly and is evidently higher than that of normal one. It is analyzed that growing blood cells in size and increasing blood viscosity make the molecular rotation of lard diet intake hindered.