Changing enzymatic conformation in organic media with pH buffer lyophilized powder

• Conformational memory of enzyme can be changed in organic media.
• The conformational rigidity of enzyme in organic phase is relative.
• The change may be influenced by the spatial redundancy of enzyme.

The conformational memory of enzyme has a significant application in biocatalysis. In this work, pH modification of tannase and the relevant factors were studied. This results indicate that direct addition of pH buffer lyophilized powder in organic media can remarkably change the conformation as did pH tunning, and the conformation variation closely correlates with the balance between hydrophilic solvents and hydrophobic solvents in reaction system. pH tunning of inner tannase and free tannase was performed. The results show that the variation may be affected by its spatial redundancy. It will be potentially applied in biocatalysis in organic media.

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