Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery

In this study, we developed a new strategy to synthesize the Tf-LNPs in a single step process based on microfluidic technology. To have a better understanding of Tf-LNPs synthesized by this single-step MF method, we put bulk mixing (BM) method as control and evaluated the Tf-LNPs produced by this single-step MF method and BM method in vitro and in vivo. The high siRNA delivery efficiency and great tumor inhibition of Tf-LNPs-MF revealed the possibility and benefit of this single-step MF method, which is a promising strategy for synthesis of targeted LNPs.180